Production and treatment of capillaries

  • Laser-Pipette-Puller P-2000
  • Pipette-Puller P 97
  • Microforge F-1000
  • Pipette Puller SA 30

Laser-Pipette-Puller P-2000

The P-2000 micropipette puller represents a significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers.

While the P-2000 is suitable for working with most conventional glasses, its primary advantage is the ability to work with quartz glass (fused silica). Quartz offers superior material properties for a variety of research applications. Quartz is stronger than other glasses and can facilitate penetration through tough tissues which would normally break conventional pipettes1 . For applications requiring a low noise glass, users will find that quartz is the lowest noise glass available2,4. Quartz contains none of the metals used in conventional glasses3. Optically, quartz is virtually free from fluorescence when illuminated.

A CO2 laser was selected as the heat source for the P-2000 for several reasons: 1) the nominal emission wavelength of the laser approximates the resonant frequency of the SiO2 lattice in glass. Thus, quartz and other conventional glasses can be melted when the appropriate laser power is supplied; 2) laser heat is clean and leaves no metal residue on the pipette as do conventional heating filaments; 3) laser heat can be turned off instantly, leaving no residual filament heat; 4) the user can program the amount and distribution of heat supplied to the glass; 5) laser heat source means there are no filaments to burn out or replace.

The P-2000 can store up to 100 separate programs, with each program consisting of up to 8 command lines. Programmable parameters include; laser power level, scan width, trip velocity, delay/ laser on time, and hard pull strength.

One important consideration for the use of the P-2000 is the diameter of the glass used. The P-2000/G is designed to produce even heating on glass up to 1.2 mm in outside diameter. Larger diameter glass can be used with the P-2000/G (up to 1.5 mm quartz and 1.8 mm conventional glass), but the performance is best with glass that is 1.2 mm diameter or less.

The P-2000/F works well with small diameter glass such as optical fibers, and with small diameter fused silica capillary commonly used for the manufacture of nanospray tips. Small diameter glass (outer diameter in the range of 0.125 mm to 0.6 mm) requires special puller bars as well as an optical alignment optimized for the smaller diameter material.

As with larger diameter glass, a wide range of tip sizes and taper geometries can be produced with the P-2000/F and small diameter glass. We have drawn optical fiber tips ranging from less than 10 nm to more than 5 µm.

Pipette-Puller P 97

The P-97 type micropipette puller is ideal for fabricating micropipettes, patch pipettes and microinjection needles. While retaining many of the features of earlier models, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. The P-97 combines a proven mechanical system with a sophisticated, programmable microprocessor controller. This programmable control of the pulling parameters allows the investigator to design application specific pipettes from a wide range of glass compositions and sizes.

A number of other features have been incorporated in the design of the P-97. Most apparent is the environmental chamber which surrounds the heating filament. This environmental chamber is designed to minimize the effect of changing humidity on the reproducibility of pulled pipettes. A 25% increase in power over the P-87 allows for the use of larger heating filaments, larger diameter glass and multi-barreled glass. The metal jaws that clamp the heating filament have also been redesigned to minimize heat retention. A gas delivery mode switch provides for extended cooling for large diameter and multi-barreled glass. A spring-loaded clamping mechanism has been added for easier loading of glass. A vacuum fluorescent display has been added that allows easy viewing.

Software improvements on the P-97 include a display of the total heat-on time to assist in program development and troubleshooting. Up to 100 programs can now be written and stored in memory, which makes the P-97 suitable for multiple users. These programs can now be write-protected, adding security to prevent programs from being changed or altered inadvertently. The display shows the last date and time the program was written or edited. In addition, the air pressure is included as a programmable parameter.

Microforge F-1000

The F-1000 microforge is used to quickly and accurately manufacture the various micro-instruments (needles, pipettes,) necessary for optimal use with all types of micro-manipulators.

Microprocessor-controlled Microforge

The F1000 is a ‘state-of-the-art’ microprocessor-controlled microforge offering unmatched performance. Designed for fabrication of both small patch clamp glass pipettes and larger injection pipettes, the F1000 should find many uses in thelaboratory. The F1000 is based on adesign similar to that first used in extremely popular microforge model. The extensive improvements incorporated into the F1000 greatly increase its versatility and performance, making it one of the most powerful microforges on the market the time that is used to achieve a desired polishing.

Digital Signal Professor Technology

In Auto mode, the heat will be applied for theduration of the timer setting. The F1000 is powered by the latestdigital signal processor (DSP) technology. Adigital timer is used to precisely control thepolish heating time. Ten memories can beused to store settings of the heating power and heating duration. All of the settings arecontrolled and displayed digitally for better accuracy and reproducibility. Two different operating modes are provided: Manual and Auto. In the Manual mode, the DSP willmemorize the duration of the timer settings.

The Pipett Puller SA 30

if allows the production of micropipettes.
Soft tube and hardened glass tube with external diameter of 0.8 to 3 mm can be used.
Other Puller on inquiry. For example: David Kopf, Sutter, etc.